h292 human lung cancer cell line nci h292  (ATCC)

Journal: Cancers

Article Title: Human Papillomavirus Oncoproteins Confer Sensitivity to Cisplatin by Interfering with Epidermal Growth Factor Receptor Nuclear Trafficking Related to More Favorable Clinical Survival Outcomes in Non-Small Cell Lung Cancer

doi: 10.3390/cancers14215333

Figure Lengend Snippet: Epidermal growth factor receptor (EGFR) expression and EGFR nuclear translocation in transfected H292-HPV16E5, H292-HPV16E6, and H292-HPV16E7 cells. ( A ) Immunoblotting to assess the efficiency of transfection and EGFR expression. Hemagglutinin (HA) was used to evaluate the transfection efficiency. B-actin was used as internal control. ( B ) Flow cytometry to assess differences in EGFR protein expression. ** p < 0.01; *** p < 0.001. ( C ) Increased phosphorylated EGFR nuclear and nonnuclear proteins after EGF 100 ng/mL stimulus for 15 min in transfected H292-HPV16E5 and H292-HPV16E6 cells. Tubulin and histone 3 were used as the internal control of nonnuclear and nuclear proteins, respectively. The uncropped blots are shown in .

Article Snippet: The human lung mucoepidermoid carcinoma cell line H292 (NCI-H292, BCRC 60372), purchased from the Bioresource Collection and Research Center (BCRC, Taiwan), was maintained in the Roswell Park Memorial Institute medium (Gibco, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin.

Techniques: Expressing, Translocation Assay, Transfection, Western Blot, Control, Flow Cytometry

Journal: Cancers

Article Title: Human Papillomavirus Oncoproteins Confer Sensitivity to Cisplatin by Interfering with Epidermal Growth Factor Receptor Nuclear Trafficking Related to More Favorable Clinical Survival Outcomes in Non-Small Cell Lung Cancer

doi: 10.3390/cancers14215333

Figure Lengend Snippet: Better treatment response in H292-HPV16E5, H292-HPV16E6, and H292-HPV16E7 cells. ( A ) Cell viability after cisplatin administration. Viability was determined using the MTT assay. ( B ) Body weight of nude mice after tumor xenografting. ( C ) Tumor volume after tumor xenografting. ( D ) Change in tumor volume after tumor xenografting. ( E ) Photographs of tumor size and comparison of tumor weight. * p < 0.05; # p < 0.05; ## p < 0.01. ( F ). Tumor inhibition rate.

Article Snippet: The human lung mucoepidermoid carcinoma cell line H292 (NCI-H292, BCRC 60372), purchased from the Bioresource Collection and Research Center (BCRC, Taiwan), was maintained in the Roswell Park Memorial Institute medium (Gibco, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin.

Techniques: MTT Assay, Comparison, Inhibition